MINI-PAM II

The new MINI-PAM-II fluorometer combines the merits of its predecessor “MINI-PAM” with most modern LED and computer technology.

Sensitivity, small dimensions, reliability under rugged conditions, and simple execution of fluorescence analysis makes the MINI-PAM-II the new standard for PAM fluorometry in field research.

Well-tested fiberoptics with 5.5 mm or 2 mm active diameter reaches even hidden samples.

Measurements under field conditions are easily controlled and monitored by a transflective touchscreen.

Energy-efficient LED sources, storage capacity of 27,000 data sets, and easy replaceable off-the-shelf batteries permit long term research campaigns at remote places.

A new fully digital leaf clip combines fluorescence analysis with measurements of photosynthetically active radiation (PAR), leaf temperature and relative humidity.

Expandable through accessories such as external multicolor lamp, optical oxygen sensor and barcode scanner.

Microsecond timing enables the MINI-PAM-II fluorometer to use the same LED as source for PAM measuring light, actinic light and saturation pulses. Measuring light corresponds to μs flashes of constant amplitude, actinic light is quasi-constant light employed to drive photosynthesis, and saturation pulses temporarily saturate primary photosynthesis so that all photosystem II reaction centers are “closed”.

Being a PAM fluorometer, the MINI-PAM-II device records only the fluorescence elicited by measuring light. Fluorescence excited by internal actinic light, saturation pulses or constant external light, like sun radiation, is not measured. Therefore, the MINI-PAM-II determines how environmental factors modulate the efficiency of conversion of measuring light into fluorescence. These “PAM fluorescence data” are required to retrieve information on primary photosynthesis like the photosynthetic efficiency of photosystem II, Y(II).

A second LED in the MINI-PAM-II emits far red light. This LED preferably excites photosystem I but is negligibly absorbed by photosystem II. A special measuring routine uses this far red LED to determine the F0’ fluorescence level which is important to correctly assess the reduction state of photosystem II reaction centers.

In experiments using internal actinic light, the light intensity at sample level can be monitored online using an internal light sensor. This internal sensor must be calibrated against an external light sensor.

The color of light emitted by the primary LED distinguishes the BLUE from the RED version of the MINI-PAM-II fluorometer (Fig. 1). The BLUE version (MINI-PAM-II/B) possesses a blue LED emitting maximally around 475 nm which is replaced by a red LED emitting maximally around 655 nm in the RED version (MINI-PAM-II/R). Both versions have a second LED providing far red light for specific excitation of photosystem I.

Figure 1: Typical LED emission spectra normalized to their maxima. The blue curve corresponds to the spectrum of the blue LED of the MINI-PAM-II/B, the orange curve represents the red LED in the MINI-PAM-II/R. Both MINI-PAM-II versions possess a far red LED which emits maximally above 700 nm (rightmost curve). Max, peak wavelength in nm. FWHM, full width at half maximum in nm.

The second difference between the two versions is the spectral window for fluorescence detection. The BLUE version detects fluorescence at wavelengths > 630 nm but the RED version detects fluorescence at wavelengths > 700 nm (Fig. 2).

Figure 2: Transmittance spectra of detection filters in the MINI-PAM-II-B (blue line) and MINI-PAM-II/R (orange line).

 

Aspects to be Considered for Selection

The detection window for fluorescence of the BLUE version extends to 640 nm but the RED version detects only fluorescence at wavelengths longer than 700 nm (Fig. 2). In principle, its extended range for fluorescence detection makes the BLUE version more sensitive than the RED version because photosystem II fluoresces at wavelength between 650 and 700 nm. In fully green leaves, however, a large part of this short wavelength fluorescence (650 - 700 nm) is reabsorbed by chlorophyll so that the gain in sensitivity of the BLUE version is moderate. When reabsorption (and also the fluorescence signal) is low, like in extremely bleached leaves, the increased sensitivity of the BLUE version can be advantageous.

The MINI-PAM-II can be used to investigate lichens or photosynthetic microbial mats. Cyanobacteria present in these mats often absorb poorly in the blue. Therefore, the RED version is normally preferred in studies of cyanobacteria.

Blue actinic light of the MINI-PAM-II/B excites the broad short wavelength band of the major light-harvesting complex of photosystem II in higher plants (LHC II). Red light of the MINI-PAM-II/R excites the comparably minor long-wavelength band of the LHC II. Hence, if LHC II excitation is important, the BLUE version is recommended.

Blue is absorbed by blue light photoreceptors which can stimulate plant responses like chloroplast relocation and stomatal movements. Therefore, the BLUE version can be advantageous when blue light responses are of interest. Blue light-driven chloroplast relocation, however, can affect the fluorescence signal by changing the efficiency of light absorption which is difficult to distinguish from the effects of high-energy fluorescence quenching on fluorescence.

 

Miniature Spectrometer MINI-SPEC/MP

The Miniature Spectrometer MINI-SPEC/MP records spectra of PAR in the visible and far red range. The MINI-SPEC/MP is, thus, well-suited to study effects of spectral variations of light on photosynthesis. The spectrometer can also be configured to measure sample reflectance which can be used as a proxy for the sample’s spectral absorption properties. Another configuration permits measurements of fluorescence emission spectra of samples where fluorescence excitation occurs either with blue or with green light. A reflectance standard and a special 160 cm cable connecting the MINI-SPEC/MP to the MINI-PAM-II are part of delivery.

Light, Temperature and Humidity Sensing Leaf Clip Holder 2035-B

The leaf clip 2035-B has been devised to record leaf temperature, light intensities at sample level and air humidity. The clip measures photosynthetically active radiation (PAR) by an LS-C Mini Quantum Sensor, a NiCr-Ni thermocouple records leaf temperature, and a capacitive type, temperature-corrected humidity sensor measures relative humidity of air. Calibration factors of PAR and temperature sensors are stored on the internal memory of the leaf clip 2035-B. A second PAR sensor can be connected to the leaf clip. Measured data are sent as digital signals to the MINI-PAM-II. To facilitate studies under field conditions, fluorescence measurements can be triggered by pushing the control button of the leaf clip 2035-B.

 

Arabidopsis Leaf Clip 2060-B

Aluminum clip with small measuring area designed to position small leaves below the fiberoptics of the MINI-PAM-II. When combined with the 2065-M Mini Quantum/Temp.-Sensor, PAR on sample level and lower leaf temperature is recorded.

 

Mini Quantum/Temp.-Sensor 2065-M

Precise mini quantum and temperature sensors usable independently or in conjunction with the 2060-B Arabidopsis Leaf Clip or the 2060-A Fiberoptics Holder for Surfaces.

 

External LED Light Source 2054-L

The 2054-L external light source complements the internal actinic light of MINI-PAM-II fluorometers. The 2054-L can be easily attached to the 2035-B leaf clip. The external lamp provides light peaking at 630 nm (red), 520 nm (green), and 452 nm (blue), as well as white light. Each of the four channels has a standard maximum photon flux density of 1500 µmol m-2 s-1; the composition of colors is freely selectable. The light source is connected to the SYNC port of the MINI-PAM-II fluorometer and it is controlled by the MINI-PAM-II or by a computer running the WinControl-3 software.

 

Magnetic Stirrer with Fiberoptics
Holder MKS-2500

The device is equipped with a specially modified stirrer plate to center and hold the KS-2500 Suspension Cuvette. The MKS-2500 Magnetic Stirrer comes with a Perspex base plate with stand bar for mounting fiberoptics on top of cuvette.

 

Fiberoptics Adapter 90º 2030-B90

The fiberoptics adapter 90º can be attached to the leaf clips 2035-B and 2060-B to position the fiberoptics af the MINI-PAM-II at 90° angle relative to leaf plane.

 

Fiberoptics Holder for Surfaces 2060-A

The holder positions the fiberoptics of the MINI-PAM-II on bulky samples. Combinable with the 2065-M Mini Quantum/Temp.-Sensor, to measure temperature and impinging PAR of the surface area investigated.

 

Miniature Fiberoptics MINI-PAM/F1

The MINI-PAM/F1 is useful when small surfaces are to be investigated. It consists of a single coated plastic fiber which provides an active diameter of 2 mm.

 

Compact Tripod ST-2101A

The tripod serves for positioning of the Leaf Clip Holder 2035-B, the Mini Quantum/Temp.-Sensor 2065-M, or the Arabidopsis Leaf Clip 2060-B.

 

Barcode Scanner BCS-9590

The barcode scanner is the ideal add-on when many differed samples are repeatedly probed. Simply mark your samples by barcodes. Then, the BCS-9590 scanner writes for each saturation pulse analysis the sample ID into the memory of the MINI-PAM-II or the report data in WinControl-3. The barcode scanner is connected to the COMP 1 or COMP 2 port of the MINI-PAM-II.